Table 5. IgM standard serial dilution in assay buffer.
Adjust the volumes depending on the plate plan and the number of plates.Initial 1:100 dilution of human IgM
| Prepare a 1:100 dilution of human IgM in the appropriate buffer prior to preparing the serial dilutions | ||||||
|---|---|---|---|---|---|---|
| Dilution | Stock (mg/mL) | Stock (µL) | Assay buffer (µL) | Total (µL) | ||
| Human IgM | 1:100 | 1.0 | 2 | 198 | 200 | |
| Serial dilution of human IgM following the initial 1:100 dilution | ||||||
| Concentration (ng/mL) | IgM Std (µL) | Assay buffer (µL) | Total (µL) | Take over (µL) | Left over (µL) | |
| Std curve human IgM (5-fold dilution) | 1,000 | 90 | 810 | 900 | 180 | 720 |
| 200 | 180 | 720 | 900 | 180 | 720 | |
| 40 | 180 | 720 | 900 | 180 | 720 | |
| 8 | 180 | 720 | 900 | 180 | 720 | |
| 1.6 | 180 | 720 | 900 | 180 | 720 | |
| 0.32 | 180 | 720 | 900 | 180 | 720 | |
| 0.006 | 180 | 720 | 900 | - | 900 | |