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. 2023 Dec 20;13(24):e4905. doi: 10.21769/BioProtoc.4905
Reagent Final concentration (%) Quantity or Volume
1× DPBS pH 7.4 94% 470 mL
Tween-20 1% 5 mL
Goat serum 5% 25 mL
Total 100% 500 mL