Skeletal muscle miR‐92b was downregulated by long‐term exercise training. (A) Reanalyzed the database obtained from GEO database (GSE95735) on human specimens from vastus lateralis before and after 12 weeks of endurance exercise (n = 3). (B) The pattern of treadmill speed for training (45 min daily for 4 weeks). (C) The qPCR was used to determine miR‐92b‐3p levels in the Gas and TA muscle from untrained and trained mice, n = 6. (D) qPCR was used to determine miR‐92b‐5p expression in Gas and TA muscle from untrained and trained mice, n = 6. (E) The qPCR was used to determine miR‐92b‐3p levels in the Gas and TA from WT mice in control (nonsuspension), unload (suspension for 7 days), and reload (7 days reload after 7 days suspension), n = 6. (F) qPCR was used to determine miR‐92b‐5p expression in Gas and TA muscle from WT mice in control (nonsuspension), unload (suspension for 7 days), and reload (7 days reload after 7 days suspension), n = 6. All results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, by unpaired Student's t test.