Figure 6.

UGP2 was a directly target of miR‐92b‐3p in skeletal muscle. (A, B) Heat map of differentially expressed genes in Gas of WT and M92KO mice (n = 3), and the Wiki Pathway Analysis of RNA‐seq on GAS, and volcano plot of differentially expressed genes in GAS of WT and M92KO mice (P < 0.05). (C) Graphical representation of the conserved miR‐92b‐3p binding motifs within the 3′‐UTR of UGP2. Complementary sequences to the seed regions of miR‐92b‐3p within the 3′‐UTRs are conserved between human (Homo) and mouse (Mus) sequences. (D) C2C12 cells were treated with miR‐92b‐3p mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of UGP2 (n = 5) and Luciferase (luc) activity of the reporter constructs containing either wild‐type ormutated (MT) 3′‐UTR of murine UGP2 after treatment of C2C12 cells with miR‐92b‐3p mimic (M92M) or Ctrl RNA (n = 5). (E) C2C12 cells treated with M92M or Ctrl RNA, the protein level of UGP2 and β‐actin were determined by western blot and the intracellular glycogen was detected by commercial kit (n = 3). (F) C2C12 cells treated with M92M and/or AdUgp2, the protein level of UGP2 and β‐actin were determined by western blot (n = 3), and the intracellular glycogen was detected by commercial kit (n = 3). All results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, by unpaired Student's t test (E to I). *P < 0.05, **P < 0.01, ***P < 0.001, by a one‐way ANOVA with a Bonferroni correction test (J, K).