Figure 1.

Dissociation of fetal human brain tissue

(A) Outline of tissue dissociation workflow.

(B) Left: typical appearance of non-intact fetal human brain tissue as received. Right: appearance of tissue after mincing.

(C) Left: Ficoll layering prior to centrifugation: upper layer contains cell suspension, and bottom layer contains Ficoll. Right: Ficoll layering after centrifugation: cells of interest are at the interface, and red blood cells have been pelleted to the bottom.

(D) Histograms of cell size distribution before and after Ficoll, on two separate donors.