Figure 2.
FACS gating strategy for human NSPCs
(A) Plot of side scatter area (SSC-A) against forward scatter area (FSC-A), gated on all events.
(B) Plot of forward scatter height (FSC-H) against forward scatter width (FSC-W), gated on “Cells.”
(C) Plot of side scatter height (SSC-H) against side scatter width (SSC-W), gated on “Singlets (FSC).”
(D) Plot of Lineage-PE against Viability-PI, gated on “Singlets (SSC).” Lineage channel consists of CD31, CD34, CD45, CD105, and CD235a.
(E) Plot of CD24-APC against THY1-FITC, gated on “Live Lineage−.”
(F) Plot of CXCR4-APC/Cy7 against EGFR-PE/Cy7, gated on “CD24−THY1−/lo.”
(G) Plot of PDGFRA-BUV395 against EGFR-PE/Cy7, gated on “THY1hi.”
(H) Plot of CXCR4-APC/Cy7 against EGFR-PE/Cy7, gated on “CD24+THY1−/lo.”
(I) List of numbered gate names, along with the enriched cell type for that gate.
(J) Graphical summary of purified neural stem and progenitor cell types, their immunophenotypes, and simplified hierarchical organization. Adapted from Liu et al., 2023.1