Figure 4.

In vivo assays for human NSPCs

(A) Positioning of pup on stereotaxic device and positioning of needle for intracerebroventricular injection (left), and visualization of successful ventricular injection by fast green dye (right).

(B) Pup before and after injection of tattoo ink back right (BR) foot (left), and appearance of back right foot tattoo after 2 months.

(C–E) Confocal immunofluorescence (IF) images of mouse brains engrafted with CD24⁻THY1^−/lo human fetal radial glia. 40 μm thick sections were stained with anti-human GFAP (C) or human cytoplasmic antigen-specific STEM121 antibody (D, E), in addition to species cross-reactive antibodies against SOX2, OLIG2, or NeuN and MAP2 (C, D, E, respectively). Abbreviations: SVZ, subventricular zone. Scale bar 25 μm. Adapted from Liu et al., 2023.¹