Mincing Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| HBSS with Ca2+/Mg2+ | 1× | 49 mL |
| HEPES (1 M) | 10 mM | 500 μL |
| DNase I (20 mg/mL) | 200 μg/mL | 500 μL |
| Total | N/A | 50 mL |
Storage: Freshly prepared. Keep on ice during experiment.
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