| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-human CD24-APC, clone 32D12 (1:25 dilution) | Miltenyi | Cat#130-095-954; RRID: AB_10829613 |
| Mouse anti-human CD90-FITC, clone 5E10 (1:50 dilution) | BioLegend | Cat#328108; RRID: AB_893429 |
| Mouse anti-human CXCR4-APC/Cy7, clone 12G5 (1:50 dilution) | BioLegend | Cat#306528; RRID: AB_2565994 |
| Mouse anti-human EGFR-PE-Cy7, clone AY13 (1:50 dilution) | BioLegend | Cat#352910; RRID: AB_2562159 |
| Mouse anti-human PDGFRA-biotin, clone 16A1 (1:50 dilution) | BioLegend | Cat#323504; RRID: AB_2162487 |
| Mouse anti-human CD31-PE, clone WM59 (1:50 dilution) | BD Biosciences | Cat#563652; RRID: AB_2738349 |
| Mouse anti-human CD34-PE, clone 581 (1:50 dilution) | BD Biosciences | Cat#562383; RRID: AB_11154586 |
| Mouse anti-human CD45-PE, clone HI30 (1:50 dilution) | BioLegend | Cat#304010; RRID: AB_314398 |
| Mouse anti-human CD105-PE, clone 266 (1:50 dilution) | BD Biosciences | Cat#562380; RRID: AB_11154054 |
| Mouse anti-human CD235a-PE, clone HIR2 (1:50 dilution) | BioLegend | Cat#306606; RRID: AB_314624 |
| BUV395 streptavidin (1:50 dilution) | BD Biosciences | Cat#564176; RRID: AB_2869553 |
| Chemicals, peptides, and recombinant proteins | ||
| Liberase | Roche | Cat#5401119001 |
| DNase I | Worthington Biochemical | Cat#LS002007 |
| Accutase | Innovative Cell Technologies | Cat#AT104 |
| Polyvinyl alcohol | Sigma | Cat#P8136 |
| Poly-L-ornithine hydrobromide | Thermo Fisher Scientific | Cat#P3655 |
| Laminin | Thermo Fisher Scientific | Cat#23017015 |
| HBSS without Ca2+/Mg2+ | Thermo Fisher Scientific | Cat#14175103 |
| HBSS with Ca2+/Mg2+ | Thermo Fisher Scientific | Cat#24020117 |
| X-VIVO 15 media | Lonza | Cat#04-744Q |
| DMEM/F12 | Thermo Fisher Scientific | Cat#11320082 |
| Hibernate-A medium | Thermo Fisher Scientific | Cat#A1247501 |
| HEPES buffer | Thermo Fisher Scientific | Cat#15630080 |
| N-2 | Thermo Fisher Scientific | Cat#17502048 |
| Heparin | STEMCELL Technologies | Cat#07980 |
| N-acetylcysteine | VWR | Cat#E-3710 |
| Fibroblast growth factor 2 (FGF) | Shenandoah Biotechnology | Cat#100-146 |
| Epidermal growth factor (EGF) | Shenandoah Biotechnology | Cat#100-26 |
| Leukemia inhibitory factor (LIF) | Sigma | Cat#LIF1010 |
| B27 | Thermo Fisher Scientific | Cat#17504044 |
| Paraformaldehyde | Electron Microscopy Sciences | Cat#15710 |
| Fast green dye | Sigma | Cat#F7252 |
| Propidium iodide | Sigma | Cat#P4170 |
| Anti-rat and anti-hamster Ig κ compensation particles | BD Biosciences | Cat#552845 |
| Anti-mouse Ig κ compensation particles | BD Biosciences | Cat#552843 |
| Experimental models: Organisms/strains | ||
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG); neonatal | The Jackson Laboratory | JAX: 005557 |
| Human: human fetal brain tissue; gestational week 16–20 | Advanced Bioscience Resources | N/A |
| Software and algorithms | ||
| ImageJ | NIH | http://wsr.imagej.net/distros/ |
| GraphPad Prism 9.0 | GraphPad Software | http://www.graphpad.com/scientific-software/prism |
| BD FACSDiva | BD Biosciences | https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdiva-software |
| FlowJo | FlowJo LLC | https://www.flowjo.com/ |
| Extreme limiting dilution analysis | Hu and Smyth2 | https://bioinf.wehi.edu.au/software/elda/ |
| Other | ||
| Stereotaxic frame | Harvard apparatus | Cat#75-1808 |
| Neonate adaptor | Stoelting | Cat#51625 |
| Microsyringe pump | World Precision Instruments | Cat#UMP3 |
| Syringe (10 μL, model 701 RN) | Hamilton | Cat#7635 |
| Needle (34 gauge, small hub RN needle, 0.375 in, point style 4 at 12°) | Hamilton | Cat#207434 |
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