Cell wash buffer

Reagent	Final concentration	Amount
Rinsing solution (autoMACS rinsing solution, Miltenyi Biotec, cat. no. 130-091-222; alternatively, use 2 mM EDTA/PBS solution)	2 mM EDTA	45 mL
Fetal bovine serum (FBS; Gibco, cat. no. 16000044)		5 mL
Penicillin-streptomycin (pen-strep; Gibco, cat. no. 1541)		0.5 mL
L-Glutamine (Gibco, cat. no. 25030)		0.5 mL
Total	N/A	50 mL

Note on storage conditions: Freshly prepare using complete IMDM with 2.5 μL of DNase added per 50 mL.

Storage conditions (keep at 4°C and maximum time for storage is 2–3 months).