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. 2023 Dec 8;4(4):102389. doi: 10.1016/j.xpro.2023.102389

PCR cycling conditions

Steps Temperature Time Cycles
Initial Denaturation 95°C 5 min 1
Denaturation 94°C 30 s 35 cycles
Annealing 60°C 30 s
Extension 72°C 1 min
Final extension 72°C 10 min 1
Hold 4°C Forever

Amplicon is 500bp.