Table 2.
cDNA synthesis reaction 2
Components | Final concentration | Amount |
---|---|---|
5x AMV Reaction Buffer | 1× | 4 μL |
RNasin Ribonuclease Inhibitor | 40 units | 0.5 μL |
AMV Reverse Transcriptase | 30 units | 1 μL |
ddH2O | 1.5 μL |
Prepare on ice. Discard unused reaction mix.