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RT-qPCR reaction
| Components | Final concentration | Amount |
|---|---|---|
| cDNA (diluted 1:5) | Maximum 10% of the final qPCR Reaction set-up volume | 4 μL |
| Maxima SYBR Green qPCR Master Mix (2×) | 1× | 6 μL |
| Forward Primer (10 μM stock) | 0.5 μM | 0.6 μL |
| Reverse Primer (10 μM stock) | 0.5 μM | 0.6 μL |
| ddH2O | Up to 12 μL |
Prepare fresh at 20°C–25°C. Discard unused reaction mix.
