Table 4.

RT-qPCR cycling conditions

Steps	Temperature	Time	Cycles
Initial Denaturation	50°C	2 min	1
Initial Denaturation	95°C	10 min	1
Denaturation	95°C	5 s	40 cycles
Annealing/Extension	60°C	30 s
Dissociation Curve	95°C	15 s	1
Dissociation Curve	60°C	15 s	1
Hold	4°C	forever