REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One Shot Stbl-3 chemically competent E. coli	Thermo Fisher Scientific	Cat# C737303
Biological samples
Leukocyte reduction chamber (LRC) from Trima apheresis collection	Vitalant Blood Donation, San Francisco	http://www.vitalant.org/
Chemicals, peptides, and recombinant proteins
Dynabeads human T-activator CD3/CD28	Life Technologies	Cat# 11131D; Lot# 00979236
AMV reverse transcriptase	Promega	Cat# M5101
Maxima SYBR Green PCR master mix	Thermo Scientific	Cat# K0253
X-tremeGENE 9 DNA transfection reagent	Roche	Cat# XTG9-RO
Critical commercial assays
Direct-zol RNA MiniPrep Kit	Zymo Research	Cat# 2052
NucleoBond Xtra Maxi Kit	Macherey-Nagel	Cat# 740414.100
eBioscience Fixable Viability Dye eFluor 780	Invitrogen	Cat# 65-0865-14; Lot# 2062571, 2450571
RosetteSep human CD4+ T cell enrichment cocktail	STEMCELL Technologies	Cat# 15062; Lot# 18A86332, 19E101792
Experimental models: Cell lines
HEK293T, human, female, epithelial kidney cells	ATCC	CRL-3216
Oligonucleotides
GFP qPCR forward primer: 5′ ATGGTGAGCAAGGGCGAGGAG 3′	Drenan et al.2	N/A
GFP qPCR reverse primer: 5′ GTGGTGCAGATGAACTTCAG 3′	Boehm et al.1	N/A
SMYD5 qPCR forward primer: AATGCACTTTATCGCTACCGAG	Boehm et al.1	PrimerBank ID: 154689857b3; https://pga.mgh.harvard.edu/primerbank/
SMYD5 qPCR reverse primer: CTGCCAACCGACATTCTGC	Boehm et al.1	PrimerBank ID: 154689857b3; https://pga.mgh.harvard.edu/primerbank/
RPL13A qPCR forward primer: GCCCTACGACAAGAAAAAGCG	Boehm et al.1	PrimerBank ID: 14591905c2; https://pga.mgh.harvard.edu/primerbank/
RPL13A qPCR reverse primer: TACTTCCAGCCAACCTCGTGA	Boehm et al.1	PrimerBank ID: 14591905c2; https://pga.mgh.harvard.edu/primerbank/
Recombinant DNA
Lentiviral packaging construct pCMVdelta R8.91	Naldini et al.3	N/A
VSV-G glycoprotein-expressing vector	Naldini et al.3	N/A
HIV-1 NL4.3-GFP	Kutsch et al.4	HIV-1 NL4.3-GFP
SMYD5 #1 TRC human shRNA	Thermo Scientific	TRCN0000155095
SMYD5 #2 TRC human shRNA	Thermo Scientific	TRCN0000156306
Non-targeting human shRNA	Millipore Sigma	SHC002
pLKO.1 mCherry	Addgene	Cat# 128073; RRID: Addgene_120873
Software and algorithms
AlphaView – AlphaImager HP 3.5.0.927	ProteinSimple	https://www.proteinsimple.com/resources
FlowJo 9.9–10.8	Tree Star	https://www.flowjo.com/
ImageJ	NIH	https://imagej.nih.gov/ij/
Prism8	GraphPad	https://www.graphpad.com/scientific-software/prism/
SDS 2.4 software	Applied Biosystems	http://home.appliedbiosystems.com
SoftMax Pro 4.7.1–6.5.1	Molecular Devices	https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-software/softmax-pro-software#gref