Recommended medium

Reagent	Final concentration	Amount
DPBS (Ca++ and Mg++-free)	N/A	48.9 mL
Fetal Bovine Serum	2%	1 mL
UltraPure 0.5 M EDTA, pH 8.0	1 mM	100 μL
Total	N/A	50 mL

The solution can be stored for up to 6 months at 4°C.