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DMEM/F12 complete medium
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM/F-12, no glutamine | N/A | 500 mL |
| Fetal Bovine Serum | 10% | 50 mL |
| L-Glutamine (200 mM) | 2 mM | 5 mL |
| Penicillin-Streptomycin (100×) | 1× | 5 mL |
| Total | N/A | 560 mL |
The medium can be stored for up to 6 months at 4°C.
