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. 2023 Dec 8;4(4):102747. doi: 10.1016/j.xpro.2023.102747

Figure 2.

Figure 2

Producing test fly populations

(A) Genetic crossing and phenotypic selection to isolate BicC transheterozygote mutants. The Punnett square depicts the progeny genotypes and viability from crossing maternal Df(2L)RA5/CyO (ΔBicC, deletion, curly wings) and either one of paternal lines BicCYC33/CyO and BicCIIF34/CyO (BicC ∗, hypomorphs, curly wings). The BicC progeny from this cross (ΔBicC/BicC∗) is straight winged.

(B) Continuous culturing to yield OreR flies or stable mutants.