Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 1;33(1):e4840. doi: 10.1002/pro.4840

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

p62/SQSTM1 interaction with LC3B. (a) Graphical representation of the AlphaScreen interaction assay. (b) Purified His‐p62/SQSTM1 A/A and GST‐LC3B interact. His‐p62 K7A K69A did not interact with LC3B, when the LIR‐motif was mutated in p62/SQSTM1. (c) LIRtide inhibits the interaction of p62 and LC3B (IC₅₀ = 1.7 μM). (d) Novobiocin inhibited the interaction of p62 and LC3B (IC₅₀ = 81 μM). Novobiocin is a small compound that binds LC3 and blocks the interaction with LIRtide. (e) Construct containing a thioredoxin domain increased interaction with LC3. (f) DTT increased the interaction of p62/SQSTM1 with LC3B. The data are representative of two independent experiments.