Table 2.
Quality assessment of IHC with CD42b.
| Formalin fixation duration | |||||
|---|---|---|---|---|---|
| 0–30 days | 30–60 days | 60–90 days | 90+ days | Overall | |
| Outcome | N | N | N | N | N (%) |
| Stainability | |||||
| Good | 20 | 19 | 20 | 20 | 79 (98.8) |
| Poor | 0 | 1 | 0 | 0 | 1 (1.2) |
| Sensitivity | |||||
| Good | 20 | 20 | 20 | 20 | 80 (100) |
| Poor | 0 | 0 | 0 | 0 | 0 |
| Specificity | |||||
| Good | 20 | 20 | 20 | 20 | 80 (100) |
| Poor | 0 | 0 | 0 | 0 | 0 |
| Consistency | |||||
| Good | 20 | 18 | 19 | 20 | 77 (96.3) |
| Poor | 0 | 2 | 1 | 0 | 3 (3.7) |
Consistency evaluated in the table refers to the similarity of staining between serial sections on same slide. Consistency between different batches and different runs is also evaluated and remained well through the entire study. IHC, immunohistochemistry.