Figure 2.

p53 knockout in epidermal keratinocytes have significant but modest effects on UVB-induced reward food intake. (A) Experimental scheme for the open field and staircase test. Wild-type mice and mice with conditional knockout of p53 in keratinocytes were habituated for 1 week followed by daily mock irradiation or 50 mJ/cm² UVB exposure for 5 weeks. At the end of the fifth week, the animals were deprived of food for 22 h and then subjected to the staircase and open field tests. (B) Number of attempts (left) and the eating scores (right) for the control and UVB-treated male mice during the staircase test. (n ≥ 10 mice per group). Data for individuals are plotted as dots and means ± SEM are shown as a bar graph. Significance was determined by two-way ANOVA analysis with student’s t-test. Details are given in Supplementary Table S2. (C) Food pellets consumed, total distance traveled (CM), time spent in center (s), total activity (%), velocity (cm/s), and time spent in periphery (s) for the control and UVB-treated male mice during the open-field test (n ≥ 11 mice per group). Data for individuals are plotted as dots and means ± SEM are shown as a bar graph. Significance was determined by two-way ANOVA analysis with Tukey’s multiple comparison (for total distance traveled, time spent in center, total activity, velocity, and time spent in periphery) and student’s t-test (for the food pellets consumed). Details are given in Supplementary Table S2. Bottom: Representative heat maps for a mouse from each group. *Represents p-value < 0.05.