Figure 3.

p53 knockout in skin epidermal keratinocytes induces anxiety-related behavior. (A) Experimental scheme for the open field test. Wild-type mice and p53-knockout mice were habituated for 1 week followed by control⁻UVB exposure for five weeks. At the end of the fifth week, the animals were starved for 22 h and then subjected to the open field test and elevated-plus maze test. (B) Total distance traveled (CM), total activity (%), time spent in the center (S), and the velocity (cm/s) in the arena were determined (n = 11 mice per group). Data for individuals are plotted as dots and means ± SEM are shown as a bar graph. Details of the statistical analyses are given in Supplementary Table S3. Bottom: Representative heat maps for males (left) and females (right) from each group. (C) Total frequency/number of visits in closed and open arms (n), activity, time spent in center (s), and velocity (cm/s) during the elevated-plus maze test. Determined (n = 11 mice per group). Data for individuals are plotted as dots and means ± SEM are shown as a bar graph. Details of the statistical analyses are given in Supplementary Table S3. Bottom: Representative heat maps for males from each group. *Represents p-value < 0.05.