FIG. 2.

Growth of E. hirae AS25 in TrisM medium. (A) Effect of KCl or NaCl on growth. KCl (200 mM) or NaCl (200 mM) was added to the cell culture at an OD₅₄₀ of 0.05, and cell growth was monitored. Symbols: ○, control; •, TrisM plus KCl; ▴, TrisM plus NaCl. (B) Effect of NaCl on growth rates (•) and the internal levels of K⁺ (○) and Na⁺ (▵) of AS25. Cells were harvested at middle logarithmic phase and washed, and the levels of K⁺ and Na⁺ were determined with an atomic absorption spectrophotometer.