KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD3 BUV737 Clone 17A2 | BD | Cat#: 612803 |
| CD4 BV605 Clone RM4-5 | BioLegend | Cat#:100548 |
| CD4 APC Clone RM4-S | BioLegend | Cat#:100516 |
| CD8 BUV 737 Clone 53-6.7 | BD | Cat#:564297 |
| CD8a PerCP-Cy5.5 Clone 53-6.7 | BioLegend | Cat#:100734 |
| CD25 BV785 Clone PC61 | BioLegend | Cat#:102051 |
| CD25 PerCP-Cy5.5 Clone PC61 | BioLegend | Cat#:102030 |
| CD44 APCe-780 Clone IM7 | eBioscience/ Thermo | Cat#:47-0441-82 |
| CD44 PerCP-Cy5.5 Clone I7 | BioLegend | Cat#:103036 |
| CD45 BUV-395 Clone 30-F11 | BD | Cat#:564279 |
| CD69 PE-Cy7 Clone H1.2F3 | BioLegend | Cat#:104510 |
| CD103 BV605 2E7 | BioLegend | Cat#:121433 |
| CD103 BV510 2E7 | BioLegend | Cat#:121423 |
| CXCL9 PE MIG-2F5.5 | BioLegend | Cat#:515604 |
| CXCR3 (CD183) PE CXCR3-173 | BioLegend | Cat#:126506 |
| CXCR3 (CD183) APC CXCR3-173 | BioLegend | Cat#:126512 |
| KLRG1 BV510 2F1/KLRG1 | BioLegend | Cat#:138421 |
| KLRG1 APC 2F1/KLRG1 | BioLegend | Cat#:138412 |
| PD1 (CD279) BV711 29F.1A12 | BioLegend | Cat#:135231 |
| CTLA-4 (CD152) BV605 UC10-4B9 | BioLegend | Cat#:106323 |
| Foxp3-e450 FJK-16S | eBioscience/ Thermo | Cat#:48-5773-82 |
| Foxp3 FITC FJK-16S | eBioscience/ Thermo | Cat#:11-5773-82 |
| T-bet PE-Cy7 4B10 | BioLegend | Cat#:644824 |
| TNFα PerCP-Cy5.5 MP6-XT22 | BioLegend | Cat#:506322 |
| IFNg e450 XMG1.2 | eBioscience/ Thermo | Cat#:48-7311-82 |
| H-2Kb MuLV p15E Tetramer-KSPWFTTL-PE | MBL | Cat#: TB-M507-1 |
| K(b)-A2/ova.SIINFEKL-APC | NIH | N/D |
| MHC Class I (H-2Kb) AF6-88.5.5.3 | BioLegend | Cat#: 17-5958-80 |
| MHC Class II (I-A/I-E) | BioLegend | Cat#: 10-7628 |
| XCR1 Alexa Fluor® 647 anti-mouse/rat XCR1 Antibody | BioLegend | Cat#: 148214 |
| Biotin anti-mouse/rat XCR1 Antibody | BioLegend | Cat#: 148212 |
| OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody | Thermo Scientist | Cat#: 13-5743-81 |
| CD16/32 antibody | BioLegend | Cat#:101320 |
| InVivoMAb anti-mouse CD8α | BioXcell | Cat#: BE0117 |
| InVivoMAb anti-mouse PD-1 (CD279) | BioXcell | Cat#: BE0146 |
| Anti-Mouse CD8b.2 Purified In vivo GOLD™ | Leinco Technologies | Cat# C2832 |
| Anti-Mouse IFN-γ Purified In vivo GOLD™ | Leinco Technologies | Cat# I-1119 |
| aCD8 alpha Clone YTS169.4 | Abcam | Cat#: ab22378 |
| Goat anti-Rat, DyLight™ 594 | Invitrogen | Cat#:PISA510020 |
| CXCL9 Recombinant Rabbit Monoclonal Antibody Clone: 11H1L14 | Invitrogen | Cat#:701117 |
| Alexa Fluor 647-conjugated bovine anti-goat IgG | Jackson Immunoresearch | Cat#: 805-607-008 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 594 | Invitrogen | Cat#:A32740 |
| Mouse CXCL10/IP-10/CRG-2 Antibody | R&D | Cat#:AF-466-NA |
| Chemicals, Peptides, and Recombinant Proteins | ||
| eBioscience Foxp3 fixation/permeabilization kit | eBioscience | Cat#:00-5523-00 |
| Tonbo Foxp3 / Transcription Factor Staining Buffer Kit | Tonbo Bioscience | Cat#:TNB-0607-KIT |
| Paraformaldehyde | Electron Microscopy Sciences | Cat# 15713S |
| DNase I | Roche | Cat#:10104159001 |
| Collagenese IV | Roche | Cat#:11088882001 |
| Diphtheria Toxin | Sigma-Aldrich | Cat#:322326 |
| DMEM High Glu w/Gl w/ Pyr | Gibco | Cat# 11995-065 |
| 100 X Penicilin-Streptomycin | Gibco | Cat# 15140-122 |
| HEPES | Gibco | Cat#: 15630-080 |
| MEM Non-Essential Amino Acids Solution (100X | Gibco | Cat#: 11140-050 |
| Fetal Bovine Serum, Heat Inactivated | Omega Scientist | Cat#: FB-01 |
| Recombinant human IL-2 Teceleukin (Tecin™) | Hoffmann-LAROCHE | NCI repository (Frederick National Laboratory for Cancer Research) |
| LEAF™ Purified anti-mouse IFN-γ [XMG1.2] | BioLegend | Cat# 505802 |
| Recombinant Mouse IL-12 Protein | R&D | Cat#: 419-ML |
| Protein Transport Inhibitor (Containing Monensin), BD GolgiStop™ | BD Biosciences | Cat#: 554724 |
| eBioscience™ Fixable Viability Dye eFluor™ 780 | eBioscience | Cat#:65-0865-14 |
| LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit | Molecular Probes | Cat#: L34962 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit | Molecular Probes | Cat# L34965 |
| ViaFluor® 405 Cell Proliferation Kit | Biotium | Cat#:30068 |
| CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry | Molecular Probes | Cat#: C34564 |
| Streptavidin, Alexa Fluor™ 647 Conjugate | Invitrogen | Cat#: S32357 |
| Triton X-100 | Fisher Scientific | Cat#: BP151-100 |
| SuperFrost Plus slides | Fisher Scientific | Cat# 12-550-15 |
| Critical Commercial Assays | ||
| CD4 Enrichment EasySep magnetic bead kit | STEMCELL Technologies | Cat#: 19852A |
| Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation | Gibco | Cat#: 11456D |
| Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA | R&D | Cat#:DY466-05 |
| Mouse CXCL9/MIG DuoSet ELISA | R&D | Cat#:DY492-05 |
| DuoSet Ancillary Reagent Kit 2 | R&D | Cat#: DY008 |
| LipoD293™ In Vitro DNA Transfection Reagent | SignaGen Laboratories | Cat#: SL100668 |
| Experimental Models: Cell lines | ||
| MC38 | Bluestone Laboratory | N/D |
| EL4 | Cell Culture facility UC Berkeley | N/D |
| 9464D | Bill Weiss Laboratory | N/D |
| MC38-OVA | Bluestone Laboratory | N/D |
| MC38-OVA-B2mKO | This paper | N/D |
| Experimental Models: Organisms/Strains | ||
| Foxp3DTR-GFP | Rudensky Laboratory | RRID:IMSR_JAX:016958 |
| CXCR3KO mice | The Jackson Laboratory | RRID:IMSR_JAX:005796 |
| Foxp3TM2-GFP | Chatila Laboratory | RRID:IMSR_JAX:006772 |
| C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Software | ||
| FlowJo (v10) | TreeStar | https://www.flowjo.com |
| Imaris Image Analysis Software | BitPlane | http://www.bitplane.com/ |
| GradhPad prism | Dotmatics | https://www.graphpad.com/scientific-software/prism/ |
| Illustrator | Adobe | https://www.adobe.com/products |