Extended Data Fig. 5. Additional data for voluntary running-induced PKA activity.
a, Representative single-cell traces of voluntary running-induced PKA response in a dSPN (top) and iSPN (bottom). b, Representative traces of integrated PKA response within an entire FOV elicited by short-duration (<30 s) voluntary running in a dSPN (top) and iSPN (bottom) overlaid with the fit for on- and off-phases (black). The entire FOV was integrated to achieve higher photon count and thereby signal-to-noise ratio, and the imaging rate was every 3 s. c, The kinetic time constant of short-duration voluntary running-induced PKA activity in dSPNs and iSPNs. n = 9 FOVs for dSPNs and 10 for iSPNs. Two-tailed Student’s t-test, from left to right dF = 17 and 17; t = 0.34 and −0.17. All error bars represent SEM and their centers represent the mean. n.s.: p > 0.05.