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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Metabolism. 2023 May 23;145:155591. doi: 10.1016/j.metabol.2023.155591

Figure 1. Intake of fructose and HFD leads to unresolved ER stress.

Figure 1.

A) Serum alanine aminotransferase (ALT) at sacrifice in male, C57BL/6J, mice drinking regular water (Chow) or 30% fructose-sweetened (Fruct) water on Chow diet or regular (HFD) or fructose water (HFD+F) on a HFD for 16 weeks. n=6–8 mice per group. B) Representative liver H&E histology, scale bar = 100 μm C) hepatic triglycerides from these mice. D) Western blot of (ATF6, IRE1α, XBP1, PERK, eIF2α, BIP, CHOP) and E) Image J quantification of their protein levels. n=3–6 per group. F) mRNA expression of ribosomal subunits (Rplp0, Rplp1, Rplp2, Rpl5), as well as G) mRNA and H) western blot of lipogenic enzymes (ACLY, ACC1, FASN, SCD1) from liver homogenates of these mice. Sample number for WB is n=4 and for mRNA n=6 mice per group. Statistically significant results based on two-way ANOVA analysis are marked with # for row factor (diet), # p<0.05; ## p<0.01; #### p<0.0001, or * for column factor (fructose water), * p<0.05; ** p<0.01. All data are presented as mean ± S.E.M.