A) Principal component analysis (PCA) of RNAseq data from the livers of mice presented in figure two. B) The most significantly altered pathways from this analysis. Volcano plot analysis in HFD+F-fed mice showing upregulated and downregulated genes with KHK knockdown (siKHK) compared to HFD+F/PBS control. Some of the most altered genes are labeled in C) and in D) are labeled the genes involved in ER stress. mRNA expression of genes regulating E) ribosomal subunits (Rplp0,Rplp1,Rplp2,Rpl5) and de novo lipogenesis (Acly, Acc1, Fasn, Scd1).