TABLE 1.

Characteristics of open reading frames identified upstream of hasA

Open reading frame	Predicted protein		Homology	Consensus motif(s)
	Size (kDa)	No. of amino acids
orf1	48.2	415	None	None
orf2	49.2	430	None	None
orf3	37.8	342	None	None
pgsA	18.5	166	pgsAa	DGXXARXXXXXXXXGXXXDXXXDb
				||  ||        |   |   |
				DGYLARKWHVVSNFGKFADPLAD
stpA	33.2	297	ABCc	GXXXXGKSd
				|    |||
				GHNGSGKS
				LSGGXXXRVXIAe
				||||   || ||
				LSGGQKQRVAIA

^aGene encoding phosphatidylglycerophosphate synthase (EC 2.7.8.5). Greatest homology is to Bacillus subtilis pgsA (25). 

^bConsensus motif for phosphatidyltransferases (19, 29). Lower line corresponds to amino acids 52 to 74 of GAS pgsA. 

^cABC transporters. Greatest homology is to the ATP binding component of a putative peptide transporter in Mycoplasma pneumoniae (18). 

^dConsensus motif for P-loop of nucleotide binding proteins (35). Lower line corresponds to amino acids 57 to 64 of GAS stpA. 

^eSignature motif for ATP binding proteins of ABC transporters (17). Lower line corresponds to amino acids 158 to 169 of GAS stpA.