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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Cell Stem Cell. 2023 Dec 7;30(12):1658–1673.e10. doi: 10.1016/j.stem.2023.11.006

Figure 1: DART-seq identifies SON as a m6A target in mouse HSCs. See also Figure S1 and Table S1S3.

Figure 1:

(A) DART-seq experimental scheme in sorted mouse HSC, MPP1, MPP2, and MPP4s.

(B) DART-seq identified significant edit sites from n=3 independent experiments. (sites with padj<0.1, differential editing > 0.1 were defined as significant sites).

(C) DART-seq identified significant target genes from n=3. independent experiments. (cut-off for significance: padj<0.1, differential editing > 0.1).

(D) DART-seq targets in mouse HSCs mainly localized to 3’UTR.

(E) De novo motif search analysis using edit sites in mouse HSCs and MPPs.

(F) Majority of HSC DART-seq targets overlap with MPP DART-seq targets.

(G) Scheme of genes whose m6A modifications increase from HSC to MPP commitment.

(H) Overlap of sites that have unique/increased m6A modification during HSC to MPP commitment with two other published datasets (m6A-seq31 and SLIMseq43).

(I) Representative track showing the C to U edit site on Son transcript and its adjacency to a DRACH motif. Editing frequencies in each sample was annotated. Yellow boxed ‘C’s were the edit sites and the sequences boxed with dotted black line were the adjacent DRACH motif. n=3 independent experiments. EV: empty vector.