Table 1 |.
Comparison of ingenuity pathway analysis top canonical pathways in mPFC, NAc and VTA tissues from SNI-Oxy and SNI-Sal animals
| Pathway | Brain region | –log(P value) | z-score | DEGs underlying pathway |
|---|---|---|---|---|
| CREB signaling in neurons | NAc | 3.61 | −2.4 | ADCY1, ADGRA1, ADGRD1, ADGRG6, ADRA2A, ATF2, CACNA1G, CYSLTR2, FGFR2, FPR3, GNB1L, GPR179, GPRC5D, HTR5A, LGR5, NMBR, NPFFR2, OXGR1, PTGFR, RRAS2, SSTR1, SSTR3 |
| mPFC | 2.04 | 0.471 | ADCY10, ADGRF4, ADGRL4, ADRB1, CACNG7, CREB5, F2RL3, FGFR2, GNAS, GNAT1, GNAT2, GNB1L, GNRHR, GPR182, GPR27, GPR3, GPR39, GPR6, GRPR, OPRM1, POLR2E, TBXA2R | |
| Hepatic fibrosis/hepatic stellate cell activation | NAc | 3 | – | ACTA2, CCN2, COL18A1, COL19A1, COL4A5, COL6A6, COL8A1, FGFR2, IL1RL2, TNFRSF1A |
| VTA | 3.42 | – | COL6A3, COL9A1, ICAM1, IL1RL2, MYH8, MYO1A | |
| Role of osteoclasts in rheumatoid arthritis signaling pathway | NAc | 2.92 | −1.155 | ADAM33, ATF2, COL18A1, COL19A1, COL4A5, COL6A6, COL8A1, FCGR2B, FRZB, IL1RL2, RRAS2, SFRP1, TNFRSF1A |
| VTA | 2.39 | −1.633 | CALCR, COL6A3, COL9A1, FOS, IL1RL2, IL7 | |
| G-protein-coupled receptor signaling | NAc | 2.77 | −2.558 | ADCY1, ADGRA1, ADGRD1, ADGRG6, ADRA2A, ATF2, CYSLTR2, FICD, FPR3, GNB1L, GPR179, GPRC5D, HTR5A, LGR5, NMBR, NPFFR2, OXGR1, PTGFR, RRAS2, SSTR1, SSTR3, TULP2 |
| mPFC | 2.43 | −1.177 | ADCY10, ADGRF4, ADGRL4, ADRB1, CNGA4, CREB5, DUSP1, EYA2, F2RL3, GNAS, GNAT1, GNAT2, GNB1L, GNRHR, GPR182, GPR27, GPR3, GPR39, GPR6, GRPR, LATS2, MAP2K3, OPRM1, PDE3A, SMPDL3B, TBXA2R | |
| VTA | 1.67 | −1.414 | BDKRB2, CALCR, FOS, GLP1R, HCAR1, MEF2C, PDE3A, RGS14 | |
| Regulation of cellular mechanics by calpain protease | NAc | 2.56 | – | CAPN11, CDK1, ITGA9, ITGAD, ITGAE, RRAS2 |
| VTA | 2.03 | – | ACTN2, CAPN11, ITGAD | |
| White adipose tissue browning pathway | NAc | 2.21 | −2.646 | ADCY1, ATF2, BDNF, CACNA1G, DIO2, FGFR2, PPARG |
| mPFC | 3.18 | 0 | ADCY10, BDNF, CACNG7, CEBPB, CREB5, FGFR2, GNAS, GUCY1A2, RXRG, THRA | |
| Wound healing signaling pathway | NAc | 2.18 | −0.632 | ACTA2, COL18A1, COL19A1, COL4A5, COL6A6, COL8A1, FGFR2, IL1RL2, RRAS2, TNFRSF1A |
| VTA | 2.1 | −1.342 | COL6A3, COL9A1, FOS, IL1RL2, LAMC2 | |
| Circadian rhythm signaling | NAc | 2 | – | ADCY1, ADCYAP1, ATF2, BDNF, CACNA1G, GNB1L, GUCY2G, NGF, RRAS2, VIP |
| mPFC | 1.95 | – | ADCY10, ADRB1, BDNF, CACNG7, CREB5, GNAS, GNB1L, GRPR, GUCY1A2, MAP2K3, MYC, RPS6KA5 | |
| Osteoarthritis pathway | NAc | 1.91 | 0 | ATF2, FRZB, IL1RL2, ITGA9, ITGAD, ITGAE, PPARG, RUNX2, TNFRSF1A |
| VTA | 2.97 | 0 | IL1RL2, ITGAD, MEF2C, PRKAG3, PTGS2, TCF7L2 | |
| Pulmonary fibrosis idiopathic signaling pathway | NAc | 1.85 | −0.905 | ACTA2, ATF2, CCN2, COL18A1, COL19A1, COL4A5, COL6A6, COL8A1, FGFR2, JARID2, RRAS2 |
| VTA | 1.66 | −1.342 | COL6A3, COL9A1, EGR1, FOS, TCF7L2 | |
| ILK signaling | mPFC | 2 | 0 | CDH1, CREB5, FLNC, LEF1, MYC, MYH4, MYO18B, MYO1H, RHOC, RPS6KA5 |
| VTA | 3.34 | −0.447 | ACTN2, FOS, KRT18, MYH8, MYO1A, PTGS2 |
DEGs underlying each pathway are bolded if they are conserved across brain regions within the same pathway. Statistics were generated in IPA.