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. Author manuscript; available in PMC: 2023 Dec 27.
Published in final edited form as: Clin Immunol. 2022 Sep 13;244:109117. doi: 10.1016/j.clim.2022.109117

Figure 3. A mast cell gene expression module classified the OA synovium into three synovial pathotypes.

Figure 3.

Total RNA was isolated from synovial tissues of OA patients who underwent total knee arthroplasty (n=40) and performed bulk RNA-Seq. (A-D) Boxplots of KIT, TPSAB1, CPA3, and HDC expression levels in the synovium of three groups of OA patients (n=40) with High, Medium or Low mast cell scores. (E) Boxplot of NEAT1 expression level as housekeeping gene in the synovium of three groups of OA patients (n=40) with High, Medium, or Low mast cell scores. The dots represent the normalized count-per-million (CPM) for each individual. NS, p>0.05; ★p≤0.05; ★★p≤0.01; ★★★p≤0.001 of one-way ANOVA with post-hoc Tukey test. (F) Heatmap of KIT, TPSAB1, CPA3, and HDC, and housekeeping genes NEAT1 expression levels across the OA patients (n=40) with High, Medium, or Low mast cell scores. The colors represent the scaled gene expression levels ranging from low (blue) to high (red) mRNA expression. Scale bar represents the scaled gene expression levels from low to high.