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. 2023 Dec 27;12:RP88795. doi: 10.7554/eLife.88795

Figure 5. Metabolic changes following Δ9-THC exposure in ESCs are transcriptionally encoded.

(A) Diagram illustrating Δ9-THC exposure scheme and experimental strategy. (B) PCA of the transcriptomics profiling of either ESCs or EpiLCs mock-exposed or exposed to 100 nM Δ9-THC. (C and D) Volcano plot in ESCs and EpiLCs, respectively, showing significance [expressed in log10(adjusted p-value or false-discovery rate, FDR)] versus fold-change [expressed in log2(fold-change, FC)]. Thresholds for significance (adjusted p-value ≤0.05) and gene expression fold-change [|(log2(FC))|>0.25 or |log2(FC)|>0.5] are shown as dashed lines. Color code is as follows: log2(FC) >0.5 in red, log2(FC) >0.25 in orange, log2(FC) >0 in light orange, log2(FC) <0 in light blue, log2(FC) >−0.25 in blue, log2(FC) >0.5 in dark blue and p-value <0.01 in pink. (E) Gene ontology (GO) terms associated with up- and downregulated DEGs [|(log2(FC)|>0.25 and p<0.01)] in ESCs and EpiLCs as determined by g:Profiler (Raudvere et al., 2019). (F) Joint pathway analysis performed by the multi-omics integration tool of MetaboAnalyst (Pang et al., 2022). The p-values were weighted based on the proportions of genes and metabolites at the individual pathway level.

Figure 5.

Figure 5—figure supplement 1. Δ9-THC exposure does not alter markers of pluripotency.

Figure 5—figure supplement 1.

Gene expression profiles of markers for the inner cell mass (ICM) and epiblast. Histograms show the median and associated errors of normalized gene counts in each condition, as indicated.
Figure 5—figure supplement 2. Δ9-THC exposure alters the expression of some epigenetic modifiers.

Figure 5—figure supplement 2.

Histograms show the median and associated errors of normalized gene counts in each condition, as indicated. Only genes with |(log2(FC)|>0.25) and p-value <0.01 from Supplementary file 1 were plotted. Statistical significance: **(p<0.01), ***(p<0.001), ****(p<0.0001).