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. 2023 Dec 27;13:22987. doi: 10.1038/s41598-023-50199-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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XRM virtual cross-sections of growth sites. The Scout & Zoom protocol coupled with the state-of-art DeepScout DL reconstruction algorithm was employed to identify smaller VOIs that correspond to the edge, corner, and planar surface fungal growth sites. These selected VOIs were rendered at higher resolution (pixel size 0.75 μm). After examining the 2D slices in the YZ plane, which depict internal cross-sections of PET fragments, it becomes evident that F2 F. oxysporum was able to penetrate deeply into PET near an edge (a), or, somewhat less extensively, near a corner (b). On the other hand, on the flat surface, there was only superficial growth (c).