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. 2023 Dec 27;13:22974. doi: 10.1038/s41598-023-49751-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of human placenta-derived mesenchymal stem cells (PL-MSCs). (a) The spindle shape morphology of PL-MSCs cultured in DMEM supplemented with 10% fetal bovine serum on day 7 after removal of non-adherent cells (left) and in passages 3 (right). (b) Flow cytometric analysis of surface marker expression in PL-MSCs showing positive expression of MSC markers (CD73, CD90, CD105) and negative expression of hematopoietic markers (CD34, CD45, HLA-DR). (c) Brilliant orange-red staining of alizarin red S in PL-MSCs on day 28 of their osteogenic differentiation. (d) Positive signal of Oil Red O staining in PL-MSCs on day 28 on their adipogenic differentiation. (e) Chondrogenic differentiation potential of PL-MSCs demonstrated by Alcian positive blue color staining of positive colonies (right) that develop in the presence of chondrogenic differentiation media. Differentiated colonies were obtained from cells of all 5 donor placentas. (a) and (c) were captured with 10X magnification. Scale bar = 100 μm. (d) was captured with 40X magnification. Scale bar = 50 μm. (e) was captured with 20X magnification. Scale bar = 100 μm.