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. 2023 Dec 20;40:101866. doi: 10.1016/j.tranon.2023.101866

Fig. 2.

Fig 2

TANs promoted tumorigenic and metastatic potential. A, schematic diagram of tumor cells/neutrophils co-culture system. Neutrophils were isolated from peripheral blood or tumor tissues, then co-cultured with Lewis cells (neutrophils: Lewis, 1:1). 48 h later, tumor cells were isolated for further assay. B, colony formation capability of Lewis pre-cultured with PBS, peripheral blood derived neutrophils and TANs respectively. C, cell migration of Lewis pre-cultured with PBS, peripheral blood derived neutrophils and TANs respectively. The scale bar was 50 μm. D, cell proliferation of Lewis pre-cultured with PBS, peripheral blood derived neutrophils and TANs respectively. E, representative images of tumor colonies (day 3) derived from CP3 and CP5. The scale bar was 50 μm. F, colony formation capability of CP3/CP5 pre-cultured with PBS, peripheral blood derived neutrophils and TANs respectively. G, cell migration of CP3/CP5 pre-cultured with PBS, peripheral blood derived neutrophils and TANs respectively.