TABLE 1.
Analysis of polymorphism at four distinct loci in different fosmids
| Fosmida | Patternb
|
|||||
|---|---|---|---|---|---|---|
| 16S RNAc | 16S-23S spacerd | 16S-GSATe
|
DNA Pole
|
|||
| HaeIII | RsaI | HaeIII | AvaII | |||
| 101G10 | A | A | A | A | A | A |
| 60A5 | B | B | B | B | B | B |
| 15A5 | B | B | — | — | b | b |
| 43H4 | A | — | — | — | A | A |
| 60H6 | A | A | — | — | a/b | B |
| 69H2 | A | — | — | — | A | A |
| 87F4 | B | — | — | — | b | a/b |
| C1H5 | A | A | A | A | ||
| C4H1 | A | A | A | A | ||
| C4H9 | A | A | A | A | A | B |
| C7D4 | A | A | A | A | A | A |
| C8B8 | B | B | B | B | B | b |
| C15A3 | A | A | A | A | ||
| C17D2 | B | — | b | B | B | b |
| C20B5 | A | A | a | a/b | ||
a
The first seven fosmids were isolated from a first library; the last eight fosmids (prefix C) are from a second library.
b
A or B, pattern identical to that of either 101G10 (=A) or 60A5 (=B); a or b, pattern similar to that of either A or B (see Materials and Methods); —, not determined. Fosmids C1H5, C4H1, C15A3, and C20B5 did not yield PCR products with polymerase (Pol)-specific primers.
c
Partial sequence (101G10 through 87F4) or RFLP analysis (C1H5 through C20B5).
d
Partial sequence.
e
RFLP analysis of PCR products.