TABLE 3.
Detection of C. symbiosum variants in natural populations of A. mexicana
A. mexicana individual or isolated DNA sourcea | Variation in 16S rDNA positionsb
|
Variation in 23S rRNA hybridizationc
|
||
---|---|---|---|---|
175 | 183.7 | Variant type A | Variant type B | |
Fosmid 101G10 from s12 | U | U | + | − |
Fosmid 60A5 from s12 | C | C | − | + |
s12 | Y | Y | + | + |
s1 | —d | — | + | + |
s2 | — | — | + | + |
s3 | Y | Y | + | + |
s4 | U | U | + | w |
s5 | Y | Y | — | — |
s6 | Y | Y | + | + |
s7 | — | — | + | w |
s8 | Y | Y | + | + |
s9 | Y | Y | + | w |
s10 | — | — | + | + |
s11 | Y | Y | + | + |
s13 | — | — | + | + |
s14 | — | — | − | w |
s16 | — | — | + | + |
s17 | — | — | − | w |
s18 | Y | Y | − | w |
s19 | — | — | + | + |
s20 | — | — | + | + |
s21 | — | — | + | + |
s22 | — | — | + | + |
s23 | — | — | + | + |
s24 | — | — | + | + |
s25 | — | — | + | + |
s26 | — | — | + | + |
s27 | — | — | + | + |
s28 | — | — | + | + |
s29 | — | — | + | − |
s30 | — | — | + | + |
hs1 | — | — | + | + |
hs2 | — | — | + | + |
hs3 | Y | Y | + | w |
hs4 | Y | Y | + | w |
hs5 | Y | Y | + | + |
hh1 | — | — | w | w |
hh2 | Y | Y | + | + |
hh3 | Y | Y | + | + |
Aq1 | Y | Y | — | — |
Aq2 | Y | Y | — | — |
Aq3 | — | — | + | + |
Prefixes: s, Naples Reef; hs, Haskle Reef; hh, Hermit Hole; Aq, captive sponge.
Y, direct sequence of PCR product yields C and U at the same position.
+, positive; −, negative; w, weakly positive.
—, not determined.