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. 2023 Dec 18;3(12):100664. doi: 10.1016/j.crmeth.2023.100664

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© 2023.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence and light scatter calibration allow EV intrainstrument comparisons

(A) rEVs acquired on the Aurora platform plotted in arbitrary units.

(B) rEVs acquired on the CytoFLEX platform plotted in arbitrary units. The green region denotes the gated area of the CytoFLEX platform in arbitrary units.

(C) Comparison of GFP intensity in arbitrary units from gated (green region in plot A-B) Aurora (solid red line) and CytoFLEX S (dotted black line) data.

(D) Comparison of SSC intensity in arbitrary units from gated (green region in plot A-B) Aurora (solid red line) and CytoFLEX S (dotted black line) data.

(E) rEVs acquired from the Aurora platform plotted in calibrated units.

(F) rEVs acquired from the CytoFLEX platform plotted in calibrated units. The green region denotes the gated area of the CytoFLEX platform in calibrated units.

(G) Comparison of GFP intensity in calibrated units from gated (green region in plot E-F) Aurora (solid red line) and CytoFLEX S (dotted black line) data as normalized probability distribution functions (pdfs).

(H) Comparison of SSC intensity in calibrated units from gated (green region in plot A-B) Aurora (solid red line) and CytoFLEX S (dotted black line) data.