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. 2023 Dec 18;3(12):100664. doi: 10.1016/j.crmeth.2023.100664

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© 2023.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Development of a small-particle detector setting optimization methodology

(A) Percentage of CV of QbSure rainbow multi-peak bead populations across a range of detector settings on the Aurora. The minimum percentage of CV per population is denoted by a black diamond.

(B) Comparison of different methods for quantification of the lower limit of detection (LLoD) on the Aurora platform. Red dotted line indicates 0.

(C and D) Aurora (C) and CytoFLEX (D) LLoD, upper LoD (ULoD), and dynamic range (DR) default instrument settings (Assay) of their FITC channel as a function of gain setting using FCM_PASS fluorescent detector optimization protocol.

(E and F) Aurora (E) and CytoFLEX (F) scatter LLoD, ULoD, and DR as a function of gain setting using FCM_PASS light scatter detector optimization protocol.