Figure 4.

405 nm light scatter detector setting incrementation

Probability density functions of buffer (gray) and rEVs (green) collected on the (A) Aurora and (B) CytoFLEX S at increasing detector settings. Probability density functions are normalized to 1 at the modal point of each distribution. The LLoD (solid red line) is specified in nm in the top right of the distribution plot and is calculated as the 99^th percentile of the buffer control. rEVs data were acquired at ∼5 × 10⁶ particles mL⁻¹ diluted in PBS. The optimal gain occurs at the lowest LoD. rEV diameter assumes a core-shell structure with a shell thickness of 5 nm, an RI of 1.486, and a core RI of 1.42.