Figure 5.

Fluorescence detector setting incrementation

Probability density functions of buffer (gray) and rEVs (green) collected on the (A) Aurora and (B) CytoFLEX S at increasing detector settings. Probability density functions are normalized to 1 at the modal point of each distribution. The LLoD (solid red line) is specified in FITC ERF in the top right of the distribution plot and is calculated as the 99^th percentile of the buffer control. rEVs data were acquired at ∼5 × 10⁶ particles mL⁻¹ diluted in PBS. The optimal gain occurs at the lowest LoD.