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. 2023 Dec 18;3(12):100664. doi: 10.1016/j.crmeth.2023.100664

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Laser power and window extension optimization

(A and B) Probability density function overlays of PBS and rEV populations at varying laser powers on the Aurora platform in (A) FITC ERF units and (B) arbitrary units.

(C and D) Summary statistics of PBS and rEV populations at varying laser powers on the Aurora platform in (C) FITC ERF units and (D) arbitrary units.

(E) LLoD of the area (black dots) and height statistics (blue dots) on Aurora and CytoFLEX S platforms with varying window extension settings. Window extension settings were altered in 1-μs increments on the Aurora from 0 to 6 μs, while the CytoFLEX S platform was compared using “default” and “high acquisition mode,” as manual adjustments to window extensions are not supported.