Figure 7.

Cross-platform standardization

(A–H) rEVs were acquired at FCM_PASS DSI derived settings on four Aurora (A–D) and four CytoFLEX S (E–H) cytometers. A rEV gate (green box) was drawn that was based on the least sensitive EGFP MESF and diameter detectable by the cohort of instruments.

(I and J) Gated rEVs were overlaid for EGFP intensity (I) and diameter (J). Boxplots using the 5^th, 25^th, 50^th, 75^th, and 95^th percentiles of each distribution were plotted for each individual cytometer above the distributions.