REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibody
anti-CD81 APC	Biolegend	Cat 349509, Lot B340367, RRID:AB_2564021
anti-CD81 Pacific Blue	Biolegend	Cat 349515, Lot B349914, RRID:AB_2687126
anti-CD81 PE	Biolegend	Cat 349505, Lot B330215, RRID:AB_10645519
Software and algorithms
MATLAB	Mathworks	https://www.mathworks.com/
FCMPASS	National Cancer Institute	https://nano.ccr.cancer.gov/fcmpass
Data analysis scripts	This paper	https://doi.org/10.6084/m9.figshare.22335835.v1
Other
Aurora + ESP (V-B-Y-R), NIH	Cytek Biosciences	N/A
Aurora + ESP (UV-V-B-Y-R), Cytek	Cytek Biosciences	N/A
Aurora + ESP (UV-V-B-Y-R), uOttawa	Cytek Biosciences	N/A
Aurora + ESP (UV-V-B-Y-R), AFC	Cytek Biosciences	N/A
CytoFLEX (V-B-Y-R), NIH	Beckman Coulter	N/A
CytoFLEX (V-B-Y-R), AFC1	Beckman Coulter	N/A
CytoFLEX (V-B-Y-R), AFC2	Beckman Coulter	N/A
CytoFLEX (V-B-Y-R), AFC3	Beckman Coulter	N/A
DI water	Sigma Aldrich	Cat 270733
DPBS	Thermo Fisher Scientific	Cat. 270733
Exosome standards, fluorescent (rEVs)	Millipore Sigma	Cat. SAE0193, Lot 125377
FluoSpheres Carnboxylate Beads (100 nm)	Thermo Fisher Scientific	F8803
Low Protein Binding Tubes (1.5 mL)	Thermo Fisher Scientific	Cat. 90410
Low Protein Binding Tubes (0.5 mL)	Thermo Fisher Scientific	Cat. 88379
MESF Beads (APC)	Becton Dickinson	Cat. 626425, Lot 0273462
MESF Beads (BV421)	Becton Dickinson	Cat. 625508, Lot 026682
MESF Beads (FITC)	Bangs Laboratories	Cat. 555B, Lot 14610
MESF Beads (PE)	Becton Dickinson	Cat 340495, Lot 51753
NIST-traceable beads (81 nm)	Thermo Fisher Scientific	Cat. 3080A, Lot 228748
NIST-traceable beads (100 nm)	Thermo Fisher Scientific	Cat. 3100A, Lot. 204935
NIST-traceable beads (152 nm)	Thermo Fisher Scientific	Cat. 3150A, Lot. 202026
NIST-traceable beads (203 nm)	Thermo Fisher Scientific	Cat. 3200A, Lot. 205131
NIST-traceable beads (240 nm)	Thermo Fisher Scientific	Cat. 3240A, Lot. 226952
NIST-traceable beads (303 nm)	Thermo Fisher Scientific	Cat. 3300A, Lot. 204665
NIST-traceable beads (345 nm)	Thermo Fisher Scientific	Cat. 3350A, Lot. 199283
NIST-traceable beads (401 nm)	Thermo Fisher Scientific	Cat. 3400A, Lot. 203859
NIST-traceable beads (453 nm)	Thermo Fisher Scientific	Cat. 3450A, Lot. 204047
Protein LoBind Tubes (5 mL)	Eppendorf	Cat. 30122356
QbSure Beads	Cytek Biosciences	Cat B7-10005, Lot AF01
V-botton plates (96-well)	Evergreen	Cat. 222-8031-01V