Table 2. Toxicity of the Selected Pyrazolo[3,4-d]pyridazine-7-one from Brine Shrimp Assay.
| 95%
fiducial limits |
|||
|---|---|---|---|
| Compd. | LD50 (μg/mL)a | Lower | Upper |
| 9b | 91.7 | 67.2 | 124.9 |
| 10b | 100.3 | 77.3 | 130.1 |
| 12 | 114.1 | 88.4 | 147.3 |
| 17a | 87.2 | 65.5 | 115.9 |
| 19a | 90.3 | 64.5 | 126.5 |
| Atropine SO4b | 737.8 | 477.4 | >1000 |
| Berberine chlorideb | 27.0 | 21.5 | 33.8 |
| ref. inhibitorc | 34.6 | 27.9 | 42.8 |
a
LD50: median lethal dose (μg/mL). LD values were estimated by probit analysis of 6-point dose (16–500 μg/mL).
b
Atropine sulfate monohydrate and berberine chloride were used as the positive controls. The solvent was used as the negative control (lower than 1% DMSO).
c
The ZIKV inhibitor (berberine derivative-4d) was described in our previous report, and it was also used as a standard.4