Figure 5.

Pirfenidone/m and ICI increase immune cell infiltration and induce immunostimulation. Quantification of (A) CD3⁺ CD4⁺ (SP, single positive) and (B) CD3⁺ CD8⁺ (SP, single positive) cells among CD45⁺ lymphocytes (n = 6–10 mice). (C) Ratio of cytotoxic CD8⁺ T cells to immunosuppressive CD4⁺ regulatory T cells (Tregs) (n = 6–10 mice). Tregs are defined as Foxp3⁺CD127^loCD25^hi CD4 SP gated on CD45⁺ lymphocytes. (D) Frequency of CD4⁺, CD8⁺, and Tregs in total lymphocyte population (n = 6–10 mice). (E) Quantification of intratumoral MDSCs (CD45⁺ CD11b⁺ GR1⁺) and (F) TAMs (CD45⁺ CD11b⁺ GR1^– F4/80⁺) gated on live cells. Percentage of antitumor (G) M1-like TAMs (CD45⁺ CD11b⁺ GR1^– F4/80⁺CD206^–CD38⁺) and (H) M2-like TAMs (CD45⁺ CD11b⁺ GR1^– F4/80⁺CD206⁺CD38^–) gated on total TAM population (n = 6–10 mice). Data are presented as mean ± SE. Statistical analyses were performed by using ordinary one-way ANOVA with multiple comparisons Dunnett test.