Skip to main content
. 2023 Sep 26;22(1):116–130. doi: 10.1111/pbi.14172

Figure 1.

Figure 1

WRKY51 is up‐regulated by RPW8.1 and feedback‐suppresses RPW8.1 expression. (a) Reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR) show the relative mRNA levels of WRKY51 in native promoter‐expressed RPW8.1 transgenic lines (R1Y4 & R1Y5) in Col‐gl background and rpw8.1 mutants in Ms‐0 and Wa‐1 background. (b) mRNA levels of WRKY51 and RPW8.1 in the transgenic lines OX51 R1Y4 (35S‐expressed WRKY51 and native promoter‐expressed RPW8.1) and the R1Y4 control. (c) Western blots for RPW8.1 protein amount in the presence or absence of WRKY51 overexpression. Protein was extracted from five‐week‐old plants for immunoblot analysis using antibodies against green fluorescent protein (GFP) (for RPW8.1) and Flag (for WRKY51) individually. ‘*’ indicates WRKY51‐Flag bands. (d) Subcellular localization of RPW8.1‐YFP (yellow fluorescent protein) in OX51 R1Y4 and R1Y4. Representative confocal images are acquired from leaves of five‐week‐old plants. YFP‐tagged RPW8.1 protein was pseudo‐colored green, and auto‐fluorescent chloroplasts were pseudo‐colored red. Scale bar = 10 μm. For (a) and (b), data are shown as mean ± SD (n = 3 independent samples). The letters above bars indicate significant differences at P < 0.01 determined by one‐way ANOVA followed by post hoc Tukey HSD analysis.