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. 2023 Sep 26;22(1):116–130. doi: 10.1111/pbi.14172

Figure 6.

Figure 6

WRKY51 directly binds to the RPW8.1 promoter. (a) Amounts of R8‐1, R8‐2 and R8‐CDS DNA pulled down by GST‐WRKY51 protein or GST control in DNA affinity purification‐quantitative polymerase chain reaction (DAP‐qPCR). PCR product amounts were normalized to actin2, R8‐1, R8‐2, R8‐CDS DNA pulled down by GST, respectively. Actin2 and R8‐CDS serve as negative controls. Data are shown as mean ± SD (n = 3 independent samples). The star above a bar indicates significant differences at P < 0.05 determined by a one‐way ANOVA followed by post hoc Tukey HSD analysis. (b–d) Binding of WRKY51 protein to probes W1, W2 and W3 in EMSA. W‐biotin: W probes labelled with biotin. Unlabeled W1 and W2 were used as competitors in fold excess to probes W1‐biotin or W2‐biotin as indicated. Wm‐competitors: competitors with mutated W‐boxes. (−) indicates absence and (+) indicates presence of the reagent.