Figure 7.

WRKY6, WRKY28 and WRKY41 repress the activity of RPW8.1 promoter. (a) Reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR) show the relative mRNA levels of 16 WRKYs in w51 R1Y4 (containing wrky51 and native promoter‐expressed RPW8.1) line and R1Y4 (containing native promoter‐expressed RPW8.1) control. The letters above bars indicate significant differences at P < 0.01 determined by one‐way ANOVA followed by post hoc Tukey HSD analysis. (b) Images of firefly luciferase (FLUC) signals expressed from the RPW8.1 promoter (R8.1 ^P ). The R8.1 ^P :FLUC reporter construct was transiently co‐expressed with construct 35S:YFP or 35S:WRKYs in Nicotiana benthamiana leaves via Agrobacterium‐mediated infiltration. LUC light signals were imaged 40 h post‐infiltration with different combinations of Agrobacterium. Renilla luciferase (RLUC) was co‐expressed as an internal reference to normalize the transfection efficiency. (c) Quantitation of firefly luminescence from (b) with different combinations of Agrobacterium concentrations. Luminescence signals were detected using a Promega GloMax 96 Luminometer. For a and c, data are shown as mean ± SD (n = 3 independent samples). The letters above bars indicate significant differences at P < 0.01 determined by one‐way ANOVA followed by post hoc Tukey HSD analysis. ‘n.s.’ indicate no significant differences.