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. 2023 Sep 26;22(1):200–215. doi: 10.1111/pbi.14180

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© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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TaMADS‐GS regulates the enrichment of H3K27me3 on TaCKX. (a) The dynamic changes of H3K27me3 pattern at four TaCKX genes in seeds at 0, 2, 4 and 6 days after pollination. (b) H3K27me3 signals on the up‐regulated genes (RNA‐seq) in Tamads‐gs compared with WT peaks. (c) H3K27me3 signals on the TaCKXs in Tamads‐gs and WT. (d) Genome browser views of H3K27me3 levels on TaCKXs in Tamads‐gs compared with wild type. (e) Reduction of H3K27me3 level in TaCKX genes that are up‐regulated in the Tamads‐gs‐d mutants. ChIP‐qPCR was performed with three biological replicates and three technical replicates for each biological replication were performed. The average values of the mutants were standardized to the average value of the WT set to one. Standard deviation is reported. Double asterisk represents statistically significant differences compared to WT and determined by Student's t‐test at P < 0.01.